Laboratory for Integrated

Micro-Mechatronic Systems

Mathieu Danoy and Stephane Poulain and Rachid Jelalli and Francoise Gilard and Sachi Kato and Charles Plessy and Taketomo Kido and Atsushi Miyajima and Yasuyuki Sakai and Eric Leclerc

The differentiation of human induced pluripotent stem cells (hiPSCs) into functional hepatocytes has the potential to solve the shortage of human primary liver cells and would be of use in drug screening. In this frame, we developed a hiPSCs maturation strategy in microfluidic biochips, using a liver-on-chip approach, a promising technology mimicking in vivo physiology. Hepato-like tissues differentiated in biochips presented advanced liver features, including ALB and CYP3A4 expressing cells. The metabolomics and transcriptomics profiles of hepato-likes cells differentiated either in biochips or Petri dishes were integrated to compare their functionalities. The multi-omics analysis revealed 41 metabolites and 302 genes differentially expressed. Overall, biochip environment lead to higher degree of hepatic differentiation demonstrated by an increase in the metabolic production of lipids, fatty acids and biliary acids …

Integration of metabolomic and transcriptomic profiles of hiPSCs-derived hepatocytes in a microfluidic environment
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